Protocol for the fast chromatin immunoprecipitation (ChIP) method

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DNA methylation presents distinct binding sites for human transcription factors (2013, methodology): “Chromatin immunoprecipitation (ChIP) was carried out in H1 cells using a rabbit anti-KLF4 antibody (H180; Santa Cruz, Dallas, TX) according to a protocol described previously (Nelson et al., 2006), except that the protein A-Sepharose was replaced with Dynabeads Protein A (Life Technologies, Grand Is”
Noncoding transcription by RNA Polymerase Pol IVb/Pol V mediates transcriptional silencing of overlapping and adjacent genes (2008, positive): “ChIP and RNA-IP ChIP was performed by adapting existing protocols (Lawrence et al., 2004; Nelson et al., 2006), as was RNA-IP (Gilbert and Svejstrup, 2006; Martianov et al., 2007).”