Barcoding diatoms: evaluation of the V4 subregion on the 18S rRNA gene, including new primers and protocols

By J. Zimmermann, R. Jahn, Birgit Gemeinholzer. Organisms Diversity & Evolution, 2011

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Data rich, information poor? Phytobenthos assessment and the Water Framework Directive (2013, background): “…future approaches will need to consider composition and new technologies such as DNA barcoding, which holds great promise (Mann et al., 2010; Zimmermann et al., 2011; Kermarrec et al., 2013) as a means of collecting basic information on composition with greater precision and lower cost than…”
DNA-based species delimitation in algae (2014, background): “Although in some cases clear discontinuities between intra- and interspecific genetic distances have been observed (e.g. Saunders, 2005; Zimmermann et al., 2011), the use of distance thresholds for species delineation may be problematic in some cases.”
Present state of the systematics of planktonic coccoid green algae of inland waters (2012, background): “The V4 subregion on the 18S rRNA gene was proposed by Zimmermann et al. (2011) for diatoms.”