Phylogenetic diversity and relationships among sorghum accessions using SSRs and RAPDs

By H. Agrama, M. Tuinstra. 2003

203 citations recorded by the source corpus.

Two DNA-based fingerprinting techniques, simple sequence repeats (SSR) and random amplified polymorphic DNA (RAPD) analyses, were applied in sorghum germplasm analysis to compare suitability for quantifying genetic diversity. Twenty-two sorghum genotypes, representing an array of germplasm sources with important agronomic traits, were assayed for polymorphism using 32 RAPD primers and 28 sets of sorghum SSR primers. The results indicated that SSR markers were highly polymorphic with an average of 4.5 alleles per primer. The RAPD primers were less polymorphic with nearly 40% of the fragments being monomorphic. An analysis of genetic diversity among sorghum lines indicated that the genetic distances calculated from SSR data were highly correlated with the distances based on the geographic origin and race classifications. Based on the results of these studies, SSR markers appear to be particularly useful for the estimation of genetic similarity among diverse genotypes of sorghum. Key words : cluster, diversity, polymorphism, RAPD, Sorghum, SSR. African Journal of Biotechnology Vol.2(10) 2003: 334-340

Subfields: Agronomy and Crop Science, Genetics

View Paper: https://www.semanticscholar.org/paper/f879e66d490a5afaedd3e22b4bb2edbac2886326

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Citations in the corpus

Assessment of genetic diversity and relationship among a collection of US sweet sorghum germplasm by SSR markers (2008, unknown): “…mean number of alleles per SSR locus (3.22) detected on 72 sorghum accessions was similar to that detected by Schloss et al. (2002) on 25 sorghum lines (3.4) but lower than that reported by Agrama and Tuinstra (2003) and Smith et al. (2000) with mean allele per locus of 4.3 and 5.9, respectively.”
An assessment of the genetic relationship between sweet and grain sorghums, within Sorghum bicolor ssp. bicolor (L.) Moench, using AFLP markers (2007, background): “49 Sureno Complex Honduras Elite tropical variety Agrama and Tuinstra (2003) –6.”
Development of SSR-based sorghum (Sorghum bicolor (L.) Moench) diversity research set of germplasm and its evaluation by morphological traits (2009, result): “But the gene diversity observed in this population is less than reported by Ali et al. (2007) i.e. 0.40 and value of 0.46, 0.62, 0.58 reported by Schloss et al. (2002), Agrama and Tuinstra (2003) and Smith et al. (2000), respectively.”
Genetic and Genomic Resources for Grain Cereals Improvement (2015, methodology): “This is supported by phylogenetic analysis using molecular markers (Tao et al., 1993; Deu et al., 1994; Cui et al., 1995; Agrama and Tuinstra, 2004; Deu et al., 2006; Perumal et al., 2007; Brown et al., 2011; Wang et al., 2013b) although this is not always the case (Ritter et al.”