Controlled expression of transgenes introduced by in vivo electroporation

By T. Matsuda, C. Cepko. Proceedings of the National Academy of Sciences of the United States of America, 2007

667 citations recorded by the source corpus.

In vivo electroporation is a powerful technique for the introduction of genes into organisms. Temporal and spatial regulation of expression of introduced genes, or of RNAi, would further enhance the utility of this method. Here we demonstrate conditional regulation of gene expression from electroporated plasmids in the postnatal rat retina and the embryonic mouse brain. For temporal regulation, Cre/loxP-mediated inducible expression vectors were used in combination with a vector expressing a conditionally active form of Cre recombinase, which is activated by 4-hydroxytamoxifen. Onset of gene expression was regulated by the timing of 4-hydroxytamoxifen administration. For spatial regulation, transgenes were expressed by using promoters specific for rod photoreceptors, bipolar cells, amacrine cells, Müller glia or progenitor cells. Combinations of these constructs will facilitate a variety of experiments, including cell-type-specific gene misexpression, conditional RNAi, and fate mapping of progenitor and precursor cells.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f80fb765d9d2ae4f611bae3cc21b7ebac516f18e

Citations in the corpus

• Wiring and Molecular Features of Prefrontal Ensembles Representing Distinct Experiences (2016, neutral): “See also Figure S4. cassette under this promoter (Kawashima et al., 2013; Li et al., 1998; Matsuda and Cepko, 2007); when injected into a reporter mouse, this viral CreER/4TM system reliably enabled activityand tamoxifen-dependent cell-body and projection labeling (Figures S4E and S4F).”