Faculty Opinions recommendation of Self versus non-self discrimination during CRISPR RNA-directed immunity.

By James C. Hu. 2010

144 citations recorded by the source corpus.

View Paper: https://www.semanticscholar.org/paper/f7a52cfca6ac7149a519e56a2dae971c67644150

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

1. Rodolphe Barrangou
2. Ambarish Biswas
3. Ryan T. Leenay
4. Peter C. Fineran
5. Joshua N. Gagnon
6. Stan J. J. Brouns
7. Kenneth R. Maksimchuk
8. Rebecca A. Slotkowski

Citations in the corpus

• C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector (2016, unknown): “Although the PFS is adjacent to the protospacer target, we chose not to use the commonly used protospacer adjacent motif (PAM) nomenclature as it has come to connote a sequence used in self vs. non-self differentiation (38), which is irrelevant in a RNA-targeting system.”
• The Biology of CRISPR-Cas: Backward and Forward. (2018, unknown): “In most type III systems, binding of the 5 0 repeat portion of the crRNA to the target inhibits DNA cleavage and serves as a PAM-independent mechanism of self-versus non-self-discrimination (Marraffini and Sontheimer, 2010).”
• CRISPRTarget (2013, background): “43 CRISPR/Cas systems have similarity, yet differences, to RNAi in mammals, which can also provide protection from viral infection.”
• Identifying and visualizing functional PAM diversity across CRISPR-Cas systems (2016, background): “…flanking sequence (called the protospaceradjacent motif or PAM) initially drives DNA interrogation by the Cas effector proteins prior to DNA unwinding and base pairing with the loaded guide RNA (Marraffini and Sontheimer, 2010; Semenova et al., 2011; Sternberg et al., 2014;Westra et al., 2012).”
• Type III CRISPR-Cas Systems: Deciphering the Most Complex Prokaryotic Immune System (2021, background): “Why do DNA targeting CRISPR Cas systems require PAMs, whereas RNA targeting systems rely on different mechanisms? In principle, the tag antitag strat egy should be suitable for discriminative DNA targeting (in fact, the tag antitag strategy of Type III systems was elaborated when these systems had b”
• Development and Applications of CRISPR-Cas9 for Genome Engineering (2014, unknown): “In type III systems, however, mismatches between the 50 end of the crRNA and the DNA target are required for plasmid interference (Marraffini and Sontheimer, 2010).”