A simple, rapid and efficient method for the extraction of genomic DNA from lychee (Litchi chinensis Sonn.)

By D. Puchooa. 2004

103 citations recorded by the source corpus.

A simple, rapid and efficient method for isolating genomic DNA from lychee (Litchi chinensis Sonn.) was developed. This modified CTAB protocol include the use of 2 M NaCl, PVP, 5% mercaptoethanol and 80% ethanol in the extraction as well as reducing the centrifugation times during the separation and precipitation of the DNA. Isolated genomic DNA showed high purity and high quantity following restriction digestion analysis.

Subfields: Genetics, Plant Science

View Paper: https://www.semanticscholar.org/paper/f5dca215bcf00820bd5cfc9fc7665f2d0519006c

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Citations in the corpus

• Genomic DNA extraction method from pearl millet (Pennisetum glaucum) leaves (2005, background): “Addition of the prewarmed, premeasured CTAB buffer to the frozen leaf tissue contained in the prechilled conical tube saves precious time in bringing the tissue from -80°C to 60°C as rapidly as possible resulting in DNA of higher quality (Puchooa, 2004).”