Polyphenolic compounds interfere with quantification of protein in soil extracts using the Bradford method

By Leonie K. Whiffen, D. Midgley, P. Mcgee. 2007

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Changes in soil aggregation and glomalin-related soil protein content as affected by the arbuscular mycorrhizal fungal species Glomus mosseae and Glomus intraradices (2009, unknown): “In addition, recent reports (Jonathan and Javier, 2006; Schindler et al., 2007; Whiffen et al., 2007) have shown that polyphenolic compounds, such as soil tannins and humic acids, may be coextracted with glomalin and interfere with the Bradford quantification.”
From peds to paradoxes: linkages between soil biota and their influences on ecological processes. (2008, background): “It may not be correlated directly with substances produced by AM hyphae (Rosier et al., 2006; Schindler et al., 2007; Whiffen et al., 2007).”
Behavior of Bradford-reactive substances is consistent with predictions for glomalin (2013, methodology): “That which is extracted using ot citric acid solutions and Bradford reactivity may partly comrise co-extracted humic acids and other proteins that survive the xtraction process (Schindler et al., 2007; Whiffen et al., 2007).”
Erratum to The chemical and functional characterization of soil N and its biotic components (2009, methodology): “However, both colorimetric (Bradford method) and immunological (ELISA) techniques can react with not only proteins but also phenolic compounds and litter and humic components (Rosier et al., 2006; Whiffen et al., 2007; Roberts and Jones, 2008).”