A simple and efficient protocol for isolation of functional RNA from plant tissues rich in secondary metabolites

By E. Kiefer, W. Heller, D. Ernst. Plant Molecular Biology Reporter, 2000

165 citations recorded by the source corpus.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/f54cda915b67165797c51c02b214d75048a58462

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In Molecular Biology

1. V. Loyola-Vargas

In Plant Science

1. V. Loyola-Vargas

Citations in the corpus

• Expression of WUSCHEL in Coffea canephora causes ectopic morphogenesis and increases somatic embryogenesis (2008, methodology): “Total RNA was obtained from 300 mg of coffee leaves following the protocol described by Kieffer and collaborators was used 300 mg of coffee leaves using extraction buffer (0.1 M Tris‐HCl (pH 8.0), 0.25 mM EDTA; 2 M NaCl; 2% CTAB (w/v); 0.5% spermidina (w/v) and 2% b-mercaptoethanol) pre-warmed at 65”