Detection of Pseudomonas syringae pv. actinidiae using polymerase chain reaction (PCR) primers based on the 16S–23S rDNA intertranscribed spacer region and comparison with PCR primers based on other…

By J. Rees-George, J. Vanneste, D. Cornish, I. Pushparajah, J. Yu, M. Templeton, K. Everett. 2010

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First report of Pseudomonas syringae pv. actinidiae causing kiwifruit bacterial canker in New Zealand (2011, unknown): “Testing of DNA extracted from cultures from leaf spots and flowers with the specific PCR primers Psa F1/R2 and Psa F3/R4 (Rees-George et al. 2010) confirmed that these isolates were Psa (Fig.”
Characterization of Pseudomonas syringae pv. actinidiae (Psa) isolated from France and assignment of Psa biovar 4 to a de novo pathovar: Pseudomonas syringae pv. actinidifoliorum pv. nov. (2015, methodology): “Three sets of primers (PsaF1/R2, Rees-George et al., 2010; KN-F/KN-R, Koh & Nou, 2002; AvrDdpx-F/AvrDdpx-R, Gallelli et al., 2011), yielding 280, 492 and 226 bp amplicons, respectively, were used to identify strains of Psa.”