Transgenic chickpea seeds expressing high levels of a bean α-amylase inhibitor

By B. K. Sarmah, A. Moore, Walter A. Tate, L. Molvig, R. Morton, D. Rees, P. Chiaiese, M. Chrispeels, L. Tabe, T. Higgins. Molecular breeding, 2004

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Achievements and Challenges in Legume Breeding for Pest and Disease Resistance (2015, background): “Alternatively, high levels of resistance to different bruchids was also shown in transgenic pea, chickpea, adzuki bean and cowpea expressing high levels of common bean α-amylase 1 inhibitor in seeds (Schroeder et al., 1995; Ishimoto et al., 1996; Sarmah et al., 2004; Solleti et al., 2008).”
Genetic engineering of chickpea (Cicer arietinum L.) with the P5CSF129A gene for osmoregulation with implications on drought tolerance (2009, background): “…involving somatic embryogenesis and organogenesis have been reported with varying success rates, asynchronous shoot bud production and low success rates for recovery of plants make these systems inefficient for genetic transformation (Kar et al. 1997; Krishnamurthy et al. 2000; Sarmah et al. 2004).”
Breeding food legumes for resistance to storage insect pests: potential and limitations. (2011, background): “Chinensis [63,64] but food safety is untested, although the α- amylase inhibitor is heat labile and denatured by cooking [38].”