Somatic Embryogenesis from 20 Open-Pollinated Families of Portuguese Plus Trees of Maritime Pine

By C. Miguel, Sónia Gonçalves, S. Tereso, L. Marum, João Maroco, M. Margarida Oliveira. Plant Cell Tissue and Organ Culture, 2004

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Biological characterization of young and aged embryogenic cultures of Pinus pinaster (Ait.) (2009, background): “Subsequent papers dealt with improvements of SE protocols to achieve higher frequencies of initiation, somatic embryo maturation, and plant production (Lelu et al. 1999; Ramarosandratana et al. 2001a,b; Miguel et al. 2004; Lelu-Walter et al. 2006).”
Plant regeneration in Stone pine (Pinus pinea L.) by somatic embryogenesis (2009, background): “These data are similar to those obtained in a broader experiment with 20 half-sib families of P. pinaster, in which the best family produced three times the overall mean and the worst was six times below the average (Miguel et al. 2004).”
Forest biotechnology: Innovative methods, emerging opportunities (2005, background): “…low initiation frequencies, has focused on modification and optimizing culture medium using immature zygotic embryos or whole immature megagametophytes (with intact developing zygotic embryos) as starting explants (Arya et al., 2000; Mathur et al., 2000; Percy et al., 2000; Miguel et al., 2004).”
Recent Progress in Somatic Embryogenesis of Four Pinus spp. (2007, methodology): “D at 13.6 μM and BA at 4.4 μM (Miguel et al. 2004).”