In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifera L.)
By Xian Yang, Z. Cao, Lizhe An, Y. M. Wang, Xiangwen Fang. Euphytica, 2006
98 citations recorded by the source corpus.
A protocol for in vitro induction of tetraploids via colchicine-treated somatic embryos from immature zygotic embryos of diploid grapevine (Vitis vinifera L.) is reported. Embryogenic callus was initiated from immature zygotic embryos cultured on Nitsch and Nitsch (NN) medium supplemented with 1.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D). The callus was transferred to NN medium containing 1.0 mg/l α-naphthalene acetic acid (NAA) and 0.5 mg/l benzyladenine (BA) to establish somatic embryogenesis. The vigorously growing globular embryos were selected and treated by 0, 10 or 20 mg/l colchicine for 1, 2 or 3 days, and then immediately transferred to NN medium supplemented with 0.03 mg/l NAA and 0.5 mg/l BA, for somatic embryo conversion and plant regeneration. The number of surviving embryos and regenerated plantlets following colchicine treatment decreased with increasing colchicine concentration and treatment time. Among 29 randomly investigated plantlets regenerated from colchicine-treated somatic embryos, five solid tetraploids (2n = 4× = 76) were identified by chromosome counting analysis; all others were diploid (2n = 2× = 38). Ploidy level of plant regenerated was also determined from leaves using flow cytometry. No chimeras with both 2C and 4C nuclei was produced from colchicine-treated somatic embryos. Significant differences in leaf stomata parameters were observed between diploid and induced tetraploid plantlets.
Subfields: Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/f2f4a49d2f8f13f50ba0c8ff303df1cc87594422
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Citations in the corpus
- Influence of ploidy level on morphology, growth and drought susceptibility in Spathiphyllum wallisii (2011, unknown): “Changes in sizes and density of the stomata after polyploidisation have also been described for many genera, e.g. C. japonica (Stanys et al. 2006), Pyrus communis (Sun et al. 2009), Vitis vinifera (Yang et al. 2006), Musa (Hamill et al. 1992) and Lagerstroemia indica (Zhang et al. 2010a).”
- Plant tissue culture: Current status, opportunities and challenges (2010, background): “, 2005), fruit species (Bouvier, 2002) or species for industrial purposes (Yang et al., 2006).”
- In vitro induction of tetraploids in crape myrtle (Lagerstroemia indica L.) (2010, background): “Several studies evaluated ploidy levels based on stomata length, width, area and frequency (Yang et al. 2006; Carvalho et al. 2005; Dwivedi et al. 1986).”
- Mitotic chromosome doubling of plant tissues in vitro (2010, methodology): “A lower density of stomata was observed in polyploids of Vitis vinifera (Yang et al. 2006), Morus alba (Chakraborti et al. 1998), Lagerstroemia indica (Zhang et al. 2010a), Pennisetum (Campos et al. 2009), among others.”
- Induction of Tetraploidy to Feverfew (Tanacetum parthenium Schulz-Bip.): Morphological, Physiological, Cytological, and Phytochemical Changes (2010, background): “The development of tetraploid feverfew may provide an avenue for the development of triploid feverfew, which may increase its productivity (Yang et al., 2006).”