5'-Deoxyribose Phosphate Lyase Activity of Human DNA Polymerase ɩ in Vitro

By K. Bebenek, A. Tissier, E. G. Frank, J. McDonald, R. Prasad, Samuel H. Wilson, R. Woodgate, Thomas A. Kunkel. Science, 2001

186 citations recorded by the source corpus.

DNA polymerase iota (pol ι) is one of several recently discovered DNA polymerases in mammalian cells whose function is unknown. We report here that human pol ι has an intrinsic 5′-deoxyribose phosphate (dRP) lyase activity. In reactions reconstituted with uracil-DNA glycosylase (UDG), apurinic/apyrimidinic (AP) endonuclease and DNA ligase I, pol ι can use its dRP lyase and polymerase activities to repair G•U and A•U pairs in DNA. These data and three distinct catalytic properties of pol ι implicate it in specialized forms of base excision repair (BER).

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/f1c8d001b869327403fcacf66cb6c8b515d94b1b

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In Cancer Research

1. B. Kaina
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Citations in the corpus

• Mechanisms of human DNA repair: an update. (2003, background): “Pol was shown to play a role in BER of G/U and A/U pairs by its intrinsic 5′-deoxyribose phosphate (dRP) lyase activity (Bebenek et al., 2001).”