Epigenetics in plant tissue culture

By M. Smulders, G. Klerk. Plant growth regulation (Print), 2011

246 citations recorded by the source corpus.

Plants produced vegetatively in tissue culture may differ from the plants from which they have been derived. Two major classes of off-types occur: genetic ones and epigenetic ones. This review is about epigenetic aberrations. We discuss recent studies that have uncovered epigenetic modifications at the molecular level, viz., changes in DNA methylation and alterations of histone methylation or acetylation. Various studies have been carried out with animals, and with plant cells or tissues that have grown in tissue culture but only little work has been done with shoots generated by axillary branching. We present various molecular methods that are being used to measure epigenetic variation. In micropropagated plants mostly differences in DNA methylation have been examined. Epigenetic changes are thought to underlie various well-known tissue-culture phenomena including rejuvenation, habituation, and morphological changes such as flower abnormalities, bushiness, and tumorous outgrowths in, among others, oil palm, gerbera, Zantedeschia and rhododendron.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/f097a540c3402541f631b0cadc6fb245cea86c50

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In Molecular Biology

1. L. Rinaldi
2. R. Petruccelli
3. A. Leva

In Plant Science

1. L. Rinaldi
2. R. Petruccelli
3. A. Leva

Citations in the corpus

• Somaclonal Variation in Tissue Culture: A Case Study with Olive (2012, background): “In addition, epigenetic changes, such as DNA methylation and histone modifications, may be associated with the physiological responses of the plant cells to the conditions in vitro [75].”