INAUGURAL ARTICLE by a Recently Elected Academy Member:Molecular architecture of the 26S proteasome holocomplex determined by an integrative approach

By Keren Lasker, F. Förster, S. Bohn, T. Walzthoeni, Elizabeth Villa, Pia Unverdorben, F. Beck, R. Aebersold, Andrej Sali, Wolfgang Baumeister. 2012

437 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Oncology

View Paper: https://www.semanticscholar.org/paper/f08300091a9c674fbbf4cc4a9a006dafc38f8c42

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Citations in the corpus

Structure and Function of the 26S Proteasome (2018, background): “The lid subcomplex acts as a scaffold that braces one side of the base (27,28) and includes six PCI (proteasome-CSN-initiation factor 3) domain–containing subunits (Rpn3, Rpn5, Rpn6, Rpn7, Rpn9, Rpn12) as well as two subunits (Rpn8 and Rpn11) with an MPN (Mpr1Pad1 N-terminal) domain (Table 1).”
The role of ubiquitination in tumorigenesis and targeted drug discovery (2020, background): “Moreover, it has been reported that the K48-type Ub chain consisting of four Ubs can be degraded by the proteasome.(494,495) Why four Ubs? These lengths of the Ub chain are also required for other linkage Ub chains.”
Integrative structure modeling with the Integrative Modeling Platform (2017, background): “13 The structure of the bacterial type II pilus was assembled from sparse NMR data and X-ray crystallographic structures of constituent proteins.”
Putting the Pieces Together: Integrative Modeling Platform Software for Structure Determination of Macromolecular Assemblies (2012, methodology): “IMP has been used to produce a number of models; for example, a eukaryotic ribosome [26], a mammalian ribosome [27], a ryanodine receptor channel [28], the 26S proteasome [1], the Hsp90 chaperonin [29], the TRiC/CCT chaperonin [30], the actin-scruin complex [31], chromatin [3], and the NPC [4].”
One number does not fit all: mapping local variations in resolution in cryo-EM reconstructions (2013, background): “Recently, other studies have explored the use of localized resolution measures to investigate the heterogeneity of a reconstruction (Bai et al., 2012; Beck et al., 2012; Lasker et al., 2012).”
Single-Particle Cryo-EM at Crystallographic Resolution. (2015, background): “…such assemblies or complexes, such as clathrin coats (Fotin et al., 2004), the 26S proteasome (da Fonseca et al., 2012; Lander et al., 2012; Lasker et al., 2012), the anaphase promoting complex (Chang et al., 2014; da Fonseca et al., 2011), and chromatin fibers (Song et al., 2014), to name…”
Protein Modelling: What Happened to the “Protein Structure Gap”? (2013, positive): “In a similar approach, the molecular architecture of the 26S proteasome holo-complex was determined (Lasker et al., 2012a).”
Template-based structure modeling of protein-protein interactions (2013, methodology): “This integrative approach has been successfully applied for the structure determination of several very large complexes [61,62].”
Go Hybrid: EM, Crystallography, and Beyond (2012, methodology): “, cross-linking/MS experiments [24] were combined with previously determined protein–protein interactions [25– 27] and crystal structures, and placed in the context of a subnanometer reconstruction of the 26S to arrive at a description of the RP subunit organization [23 ].”