A laboratory study of producing docosahexaenoic acid from biodiesel-waste glycerol by microalgal fermentation

By Zhanyou Chi, D. Pyle, Z. Wen, C. Frear, Shulin Chen. 2007

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Heterotrophic cultures of microalgae: metabolism and potential products. (2011, background): “…source of these economically-important fatty acids, especially under heterotrophic conditions that reduced the costs of production (Barclay et al., 1994; Vazhappilly and Chen, 1998; Apt and Behrens, 1999; Wen and Chen, 2003; Sijtsma and de Swaaf, 2004; Sijtsma et al., 2005; Chi et al., 2007).”
Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products (2013, methodology): “51 g/L/day using waste glycerol as substrate [74].”
Value-added uses for crude glycerol--a byproduct of biodiesel production (2012, neutral): “91 g/L under the optimized culture conditions [58].”
Microalgae flocculation: Impact of flocculant type, algae species and cell concentration (2014, background): “05–18 g/L) [2,7].”