A Characterization of the DNA Data Storage Channel

By Reinhard Heckel, Gediminas Mikutis, R. Grass. Scientific Reports, 2018

318 citations recorded by the source corpus.

Owing to its longevity and enormous information density, DNA, the molecule encoding biological information, has emerged as a promising archival storage medium. However, due to technological constraints, data can only be written onto many short DNA molecules that are stored in an unordered way, and can only be read by sampling from this DNA pool. Moreover, imperfections in writing (synthesis), reading (sequencing), storage, and handling of the DNA, in particular amplification via PCR, lead to a loss of DNA molecules and induce errors within the molecules. In order to design DNA storage systems, a qualitative and quantitative understanding of the errors and the loss of molecules is crucial. In this paper, we characterize those error probabilities by analyzing data from our own experiments as well as from experiments of two different groups. We find that errors within molecules are mainly due to synthesis and sequencing, while imperfections in handling and storage lead to a significant loss of sequences. The aim of our study is to help guide the design of future DNA data storage systems by providing a quantitative and qualitative understanding of the DNA data storage channel.

Subfields: Mechanical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ee96d4f9b3f98f7f1df19c7de441b05e0892021e

Citations in the corpus

• Molecular digital data storage using DNA (2019, unknown): “Heckel et al. have characterized the coding channel further 34 by analysing results from three previous studies 6,20,32 , showing that errors mostly stem from synthesis and sequencing and that DNA manipulation, PCR and storage may cause erasures , that is, certain sequences becoming…”