Zika virus impairs growth in human neurospheres and brain organoids
By P. Garcez, E. Loiola, R. F. Madeiro da Costa, L. Higa, P. Trindade, R. Delvecchio, J. Nascimento, R. Brindeiro, A. Tanuri, S. Rehen. Science, 2016
1,118 citations recorded by the source corpus.
Zika virus tested in human brain organoids The pernicious and resilient Aedes mosquito is rapidly spreading Zika virus (ZIKV) through the Americas. ZIKV infection mostly causes mild disease, but in some patients, nervous system involvement is indicated. A particular worry is an observed correlation between infection of mothers in the first trimester of pregnancy and microcephaly in newborns. Garcez et al. tested the effects of ZIKV compared with dengue virus infection on human neural stem cells grown as organoids. ZIKV targeted the human brain cells, reduced their size and viability in vitro, and caused programmed cell death responses. Science, this issue p. 816 Zika virus infection in cell culture models damages human neural stem cells to limit growth and cause cell death. Since the emergence of Zika virus (ZIKV), reports of microcephaly have increased considerably in Brazil; however, causality between the viral epidemic and malformations in fetal brains needs further confirmation. We examined the effects of ZIKV infection in human neural stem cells growing as neurospheres and brain organoids. Using immunocytochemistry and electron microscopy, we showed that ZIKV targets human brain cells, reducing their viability and growth as neurospheres and brain organoids. These results suggest that ZIKV abrogates neurogenesis during human brain development.
Subfields: Public Health, Environmental and Occupational Health
View Paper: https://www.semanticscholar.org/paper/9758c16ecc871315ee1b4591aaa71471e7e47be4
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In Public Health, Environmental and Occupational Health
Citations in the corpus
- Zika Virus Disrupts Neural Progenitor Development and Leads to Microcephaly in Mice. (2016, background): “ZIKV infection induces cell death and deregulation of cell-cycle progression of hiPSCs, reducing their viability andgrowth as neurospheres andbrain organoids (Garcez et al., 2016; Tang et al., 2016, Qian et al., 2016).”
- Brain organoids: advances, applications and challenges (2019, background): “For example, exposure of brain organoids to Zika virus results in preferential infection of NPCs, which suppresses proliferation and causes an increase in cell death, ultimately leading to drastically reduced organoid size (Cugola et al., 2016; Dang et al., 2016; Garcez et al., 2016; Qian et al., 20”
- Brain Region-specific Organoids using Mini-bioreactors for Modeling ZIKV Exposure (2016, positive): “Recent studies of human NPCs in 2D and neurosphere cultures showed efficient infection by ZIKV, leading to increased cell death and attenuated growth (Tang et al., 2016) (Garcêz et al. 2016, PeerJ, preprint).”