Temperature sensing using fluorescent nanothermometers.

By F. Vetrone, Rafik Naccache, A. Zamarrón, Ángeles Juarranz de la Fuente, F. Sanz‐Rodríguez, Laura Martinez Maestro, Emma Martín Rodríguez, D. Jaque, José García Solé, J. Capobianco. ACS Nano, 2010

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Acquiring the temperature of a single living cell is not a trivial task. In this paper, we devise a novel nanothermometer, capable of accurately determining the temperature of solutions as well as biological systems such as HeLa cancer cells. The nanothermometer is based on the temperature-sensitive fluorescence of NaYF(4):Er(3+),Yb(3+) nanoparticles, where the intensity ratio of the green fluorescence bands of the Er(3+) dopant ions ((2)H(11/2) --> (4)I(15/2) and (4)S(3/2) --> (4)I(15/2)) changes with temperature. The nanothermometers were first used to obtain thermal profiles created when heating a colloidal solution of NaYF(4):Er(3+),Yb(3+) nanoparticles in water using a pump-probe experiment. Following incubation of the nanoparticles with HeLa cervical cancer cells and their subsequent uptake, the fluorescent nanothermometers measured the internal temperature of the living cell from 25 degrees C to its thermally induced death at 45 degrees C.

Subfields: Electrical and Electronic Engineering, Materials Chemistry

View Paper: https://www.semanticscholar.org/paper/339643ad0e1b06e3c6c87cd978473b918eaf90fd

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Temperature sensing using fluorescent nanothermometers.

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