Optimization of Procedures for Counting Viruses by Flow Cytometry

By C. Brussaard. Applied and Environmental Microbiology, 2004

650 citations recorded by the source corpus.

Subfields: Ecology

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In Ecology

Citations in the corpus

Lack-of P-limitation of phytoplankton and heterotrophic prokaryotes in surface waters of three anticyclonic eddies in the stratified Mediterranean Sea (2011, background): “0.5% for the former and 1% for the latter), frozen in liquid nitrogen, and stored at−80◦C until analysis (e.g., Troussellier et al., 1995; Marie et al., 2000; Lebaron et al., 2001; Brussaard, 2004).”
Carbohydrate production by phytoplankton and degradation in the marine microbial food web (2006, methodology): “Prokaryotes were detected by their signature in a plot of side scatter (SSC) versus green fluorescence (FL1) as described elsewhere (Gasol and Del Giorgio 2000; Brussaard 2004).”
Viruses as mortality agents of picophytoplankton in the deep chlorophyll maximum layer during IRONAGES III (2007, methodology): “…of viruses was determined on glutaraldehyde-fixed samples (final concentration, 0.5% glutaraldehyde, frozen in liquid nitrogen and stored at 280uC prior to analysis) with a Becton-Dickinson FACSCalibur flow cytometer, with 15 mW of 488-nm aircooled argon-ion laser according to Brussaard (2004b).”
Enumeration of virus particles in aquatic or sediment samples by epifluorescence microscopy (2010, result): “Many earlier estimates of viral abundance have been made on preserved samples; however, several studies have shown rapid and significant decay of viral particles in aldehyde-fixed samples (Brussaard 2004; Danovaro et al. 2001; Wen et al. 2004), although there is evidence that the decay is lessened w”
Abundance and activity of major groups of prokaryotic plankton in the coastal North Sea during spring and summer (2006, methodology): “Mar Ecol Prog Ser 123:259–271 Brussaard CPD, Gast GJ, Van Duyl FC, Riegman R (1996) Impact of phytoplankton bloom magnitude on a pelagic microbial food web.”
Comparison of SYBR Green I and SYBR Gold stains for enumerating bacteria and viruses by epifluorescence microscopy (2006, methodology): “It is also notable that decreases in viral counts could be prevented by similar storage procedures prior to enumeration with either epifluorescence microscopy (Wen et al. 2004) or flow cytometry (Brussaard 2004).”
Viral control of bacterial growth efficiency in marine pelagic environments (2009, methodology): “2-mm filtered TE buffer (pH 8; viral contamination was negligible), stained with SYBR Green I (1 : 25,000 dilution of commercial stock; Molecular probes) for 10 min at room temperature (for bacteria) or at 80uC (for viruses), and analyzed with a flow cytometer (FACSCalibur; Becton Dikinson) equipped”
Viruses in the sea (2005, background): “, although flow cytometry shows promise as a high-throughput metho”
Optimized routine flow cytometric enumeration of heterotrophic flagellates using SYBR Green I (2011, methodology): “…Li 1994; Marie et al. 2000), and of heterotrophic bacteria (Robertson and Button 1989; Monger and Landry 1992; Li et al. 1995, Marie et al. 1996; del Giorgio et al. 1996; Lebaron et al. 1998; Troussellier et al. 1999) and viruses (Marie et al. 1999; Brussaard 2004) using a variety of fluorochromes.”
Viriobenthos in freshwater and marine sediments : a review (2008, methodology): “In the attempt to decrease sample-processing time, the use of flow cytometers has been proposed for detecting and quantifying virus-like particles and prokaryotes (Marie et al., 1999; Brussaard, 2004).”
Tangential flow diafiltration: an improved technique for estimation of virioplankton production (2005, methodology): “Mar Ecol Prog Ser 83:273–280 Brussaard CPD (2004) Optimization of procedures for count- ing viruses by flow cytometry.”