HaloTag: a novel protein labeling technology for cell imaging and protein analysis.

By G. Los, L. Encell, Mark G Mcdougall, Danette D Hartzell, N. Karassina, C. Zimprich, Monika G. Wood, R. Learish, R. Ohana, M. Urh and 10 more. ACS Chemical Biology, 2008

2,182 citations recorded by the source corpus.

Subfields: Cell Biology, Genetics, Organic Chemistry

View Paper: https://www.semanticscholar.org/paper/ed764f535292533bd0ec3e0ed9f48760c46ddeaf

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Citations in the corpus

Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications (2012, background): “The versatility associated with the HaloTag® includes a variety of ligands applicable for protein visualization, trafficking, and turnover [156, 157], as well as analysis of protein interactions [156].”
Nuclear pores as versatile reference standards for quantitative superresolution microscopy (2019, neutral): “Intracellular labeling with SNAP-tag or HaloTag did not result in complete labeling as is possible in vitro 20,21 .”
A general method to improve fluorophores for live-cell and single-molecule microscopy (2015, neutral): “Although previous work had shown the pyrrolidine-containing rhodamine 5 to have a higher fluorescence brightness than the piperidino-rhodamine 6 , 25,28 azacyclic-rhodamines with smaller or larger ring sizes ( 3 , 4 , and 7 ) had not been explored.”
CAVER 3.0: A Tool for the Analysis of Transport Pathways in Dynamic Protein Structures (2012, background): “K175M+C176G+Y273L 10,000-times higher binding rate of fluorescent probe [63]”
Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading (2020, methodology): “Due to the superior fluorescent properties of organic dyes over fluorescent proteins and the possibility of flexible pulse labeling, a HaloTag approach was chosen for fluorescent labelling of the BglG protein for live cell and super45 resolution imaging (31).”
Single-Cell and Single-Molecule Analysis of Gene Expression Regulation (2016, background): “Especially remarkable for its versatility is the HaloTag technology, a modular tag system based on a modified haloalkane dehalogenase (Halo) from bacteria designed to covalently bind to synthetic ligands (94).”
OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization (2022, background): “Solutions to this obstacle include using FP repeats to increase signal ( 18 , 23 ) or using tags that bind chemical fluorophores [e.g., HaloTag ( 75 )], which can be brighter than FPs or operate at wavelengths where cellular autofluorescence is decreased ( 76 ).”
Proteolysis Targeting Chimeras as Therapeutics and Tools for Biological Discovery (2020, positive): “The HaloPROTAC system utilizes the HaloTag® protein, an engineered bacterial dehalogenase, which allows the orthogonal, covalent conjugation of a chloroalkane-labelled molecule to a fusion protein of interest (Los et al., 2008).”