標的類似体と結合したCRISPR-Cas系Cmr複合体の結晶構造;標的類似体と結合したCRISPR-Cas系Cmr複合体の結晶構造;Crystal Structure of the CRISPR-Cas RNA Silencing Cmr Complex Bound to a Target Analog

By T. Numata, T. Osawa. 2015

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CRISPR-Cas constitutes a prokaryotic adaptive immune system against invading genetic elements. The crRNA and Cas protein （ s ） form effector complex that degrades invading nucleic acid complementary to the crRNA guide. The type III-B Cmr effector complex comprises six Cas proteins and a crRNA, and degrades RNA target. The crystal structure of the Cmr complex bound to a target analog was determined. The complex recognizes the crRNA 5 ʼ -tag and deforms the guide-target duplex at 6-nt intervals. The structure reveals the periodic RNA cleavage mechanism by the Cmr complex, and provides insights into the evolution of the type I and III effector complexes.

Subfields: Cardiology and Cardiovascular Medicine, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/d904aa3f14be94861a071bfdfa3964ea7c025b12