OMIP-042: 21-color flow cytometry to comprehensively immunophenotype major lymphocyte and myeloid subsets in human peripheral blood

By Karl W Staser, William C. Eades, Jaebok Choi, D. Karpova, John F DiPersio. 2017

58 citations recorded by the source corpus.

THIS 21-color flow cytometry-based OMIP (1) enables simultaneous quantification of monocytes, basophils, granulocytes, dendritic cells, natural killer cells, B cells, and all well-defined T and T helper cell subsets in the human peripheral blood (Table 1). This panel captures the major phenotypes described in the NIH Human Immunology Project (2,3) with additional markers for deep T cell analysis (4). We specifically designed this panel for analysis of peripheral blood from patients involved in our clinical trials of novel agents for the treatment of graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (alloHSCT). We have optimized this panel for the analysis of 1 3 10 fresh or previously frozen peripheral blood mononuclear cells (PBMCs).

Subfields: Hematology, Immunology

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In Immunology

1. Josef Spidlen
2. Richard L. Halpert
3. Chris Ciccolella
4. R. Anno
5. J. Snyder-Cappione
6. Anna C. Belkina

Citations in the corpus

• Automated Optimal Parameters for T-Distributed Stochastic Neighbor Embedding Improve Visualization and Allow Analysis of Large Datasets (2018, background): “With recent advances in instrumentation and reagent availability, flow cytometry datasets with >20 parameters are quickly becoming prevalent and even standard in the field 29-31 , yet the proper data assessment tools are lacking for general use.”