An efficient one-step site-directed and site-saturation mutagenesis protocol.

By Lei Zheng, U. Baumann, Jean-Louis Reymond. Nucleic Acids Research, 2004

1,175 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Site-Directed Mutagenesis (2017, background): “it helps to introduce precise genetic variations at specific sites in a PCR based process and the mutant construct is then cloned into the desirable vector system [2].”
A rapid, efficient, and economical inverse polymerase chain reaction-based method for generating a site saturation mutant library. (2014, positive): “The above libraries were generated using diverse techniques including polymerase chain reaction (PCR)-like linear amplification with partially overlapping primer [13,19] or a megaprimer-based approach [10,20] or by overlap extension PCR [5,16,18,21].”
Functional and molecular characteristics of rice and barley NIP aquaporins transporting water, hydrogen peroxide and arsenite (2014, positive): “Point mutation of the construct for the amino acid substitution was done essentially using the one-step site-directed and sitesaturation mutagenesis protocol according to the previous report (Zheng et al. 2004).”