Control of leaf vascular patterning by polar auxin transport.

By Enrico Scarpella, Danielle Marcos, Jir□í Friml, T. Berleth. Genes & Development, 2006

826 citations recorded by the source corpus.

The formation of the leaf vascular pattern has fascinated biologists for centuries. In the early leaf primordium, complex networks of procambial cells emerge from homogeneous subepidermal tissue. The molecular nature of the underlying positional information is unknown, but various lines of evidence implicate gradually restricted transport routes of the plant hormone auxin in defining sites of procambium formation. Here we show that a crucial member of the AtPIN family of auxin-efflux-associated proteins, AtPIN1, is expressed prior to pre-procambial and procambial cell fate markers in domains that become restricted toward sites of procambium formation. Subcellular AtPIN1 polarity indicates that auxin is directed to distinct "convergence points" in the epidermis, from where it defines the positions of major veins. Integrated polarities in all emerging veins indicate auxin drainage toward pre-existing veins, but veins display divergent polarities as they become connected at both ends. Auxin application and transport inhibition reveal that convergence point positioning and AtPIN1 expression domain dynamics are self-organizing, auxin-transport-dependent processes. We derive a model for self-regulated, reiterative patterning of all vein orders and postulate at its onset a common epidermal auxin-focusing mechanism for major-vein positioning and phyllotactic patterning.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/ea6d2e20ee2659b7162ddd023a0ab644bda04b74

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Jir□í Friml
2. René Benjamins
3. Ben Scheres
4. J. Petrášek
5. Marcus Heisler
6. M. Estelle
7. C. Luschnig
8. C. Ohno

In Plant Science

1. Jir□í Friml
2. L. Sack
3. C. Scoffoni
4. Tim J. Brodribb
5. René Benjamins
6. Ben Scheres
7. T. Feild
8. J. Petrášek

Citations in the corpus

• Leaf Maximum Photosynthetic Rate and Venation Are Linked by Hydraulics1[W][OA] (2007, background): “…and developmental processes that guide leaf vein development and patterning grows at an ever-increasing rate (Kang and Dengler, 2004; Fleming, 2005; Scarpella et al., 2006; Sieburth and Deyholos, 2006), the underlying physical principles that connect vein pattern with leaf productivity remain…”
• Model for the regulation of Arabidopsis thaliana leaf margin development (2011, background): “PIN1 convergence points in the leaf epidermis not only are required for serration patterning, but also mark sites where auxin is transported to internal tissue layers and guides the development of vasculature (4, 6).”
• Antagonistic regulation of PIN phosphorylation by PP2A and PINOID directs auxin flux. (2007, background): “Furthermore, PIN localization can be modulated by environmental (e.g., gravity) or developmental cues (Friml et al., 2002b, 2003; Benková et al., 2003; Reinhardt r Inc. et al., 2003; Scarpella et al., 2006).”
• Auxin: the looping star in plant development. (2008, methodology): “Another model included a variable that was called the auxin flux-bifurcator, which makes it possible that a given cell under specific conditions can transport auxin in two directions, fitting the data of Scarpella and coworkers (34, 105).”
• The plant vascular system: evolution, development and functions. (2013, background): “Specifically, the initial broad expression domain of auxin in the developing leaf converges and tapers to narrow cell files (presumptive vascular strands) that are dependent on auxin transport (Scarpella et al. 2006; Sawchuk et al. 2008).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “The transcript abundance was normalized against peanut Ubiquitin (Luo et al. , 2005) transcript levels.”
• Leaf venation: structure, function, development, evolution, ecology and applications in the past, present and future. (2013, background): “…(Nikolopoulos et al., 2002; Liakoura et al., 2009); Kleaf and its dynamics with irradiance and leaf water status (Zwieniecki et al., 2007; Scoffoni et al., 2008; Sommerville et al., 2012); stomatal control (Terashima, 1992; Buckley et al., 2011); and biomechanical support (Haberlandt,…”
• Auxin receptors and plant development: a new signaling paradigm. (2008, background): “In leaves, as in embryos, expression of ARF5 precedes vascular development (Scarpella et al. 2006, Weijers et al. 2006, Wenzel et al. 2007).”
• Auxin: a trigger for change in plant development. (2009, neutral): “ment, root meristem patterning, organogenesis, and vascular tissue differentiation (Luschnig et al., 1998; Friml et al., 2002a, 2002b, 2003; Benková et al., 2003; Reinhardt et al., 2003; Blilou et al., 2005; Scarpella et al., 2006).”
• Auxin transport routes in plant development (2009, background): “For example, during the formation of vascular veins in leaves, PIN1 directs auxin towards a convergence point in the leaf epidermis, from where veins are being initiated and where PIN1 expression and polar localization mark the position of all future veins (Scarpella et al., 2006) (Fig.”