Comprehensive Analysis of Silencing Mutants Reveals Complex Regulation of the Arabidopsis Methylome

By Hume Stroud, Maxim V. C. Greenberg, Suhua Feng, Yana V. Bernatavichute, Steven E. Jacobsen. Cell, 2013

802 citations recorded by the source corpus.

SUMMARY Cytosine methylation is involved in various biological processes such as silencing of transposable elements (TEs) and imprinting. Multiple pathways regulate DNA methylation in different sequence contexts, but the factors that regulate DNA methylation at a given site in the genome largely remain unknown. Here we have surveyed the methylomes of a comprehensive list of 86 Arabidopsis gene silencing mutants by generating single-nucleotide resolution maps of DNA methylation. We find that DNA methylation is site specifically regulated by different factors. Furthermore, we have identified additional regulators of DNA methylation. These data and analyses will serve as a comprehensive community resource for further understanding the control of DNA methylation patterning.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/ea3a75d8e4c991df0790138a26308fa5726c12ab

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Plant Science

1. Jiaxu Li
2. S. Sah
3. K. Raja Reddy

Citations in the corpus

• Abscisic Acid and Abiotic Stress Tolerance in Crop Plants (2016, background): “While CG methylation can occur in active genes, CHG and CHH methylation is almost exclusively present in heterochromatin (Stroud et al., 2013).”
• Epigenomic diversity in a global collection of Arabidopsis thaliana accessions (2016, neutral): “Surprisingly, AGO1 and AGO9 were associated with genomewide average mCHH levels, given that knockout of either locus does not affect average mCHH levels within RdDM-target regions (Stroud et al., 2013).”