Enhanced sensitivity of DNA- and rRNA-based stable isotope probing by fractionation and quantitative analysis of isopycnic centrifugation gradients.

By T. Lueders, M. Manefield, M. Friedrich. Environmental Microbiology, 2003

650 citations recorded by the source corpus.

Subfields: Ecology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/ea0894f17d24c2af5cd9d2eb4fdfa414c0047e29

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In Ecology

1. J. Prosser
2. Z. Jia
3. P. Claus
4. R. Conrad
5. P. Hirsch
6. I. Clark
7. T. Mauchline
8. Ji‐Zheng He

In Molecular Biology

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Citations in the corpus

• Identification of in situ 2,4-dichlorophenoxyacetic acid-degrading soil microorganisms using DNA-stable isotope probing (2007, neutral): “…fractions (150 ml) were collected at the bottom as previously described (Cupples et al., 2006; Lueders et al., 2004b), the BD of each fraction was then determined, CsCl was removed (Cupples et al., 2006; Griffiths et al., 2000; Lueders et al., 2004b) and purified DNA fractions were stored at 20 1C.”
• Culture-independent molecular techniques for soil microbial ecology (2010, background): “For example, a range of 13C substrates have been used to probe different groups in soil using both DNA and RNA-SIP (Manefield et al., 2002; Lueders et al., 2004; Whiteley et al., 2006).”
• Plant host habitat and root exudates shape soil bacterial community structure (2008, methodology): “Extracted DNA was fractionated by CsCl equilibrium density-gradient centrifugation (Lueders et al., 2004; Haichar et al., 2007).”
• Reduced dependence of rhizosphere microbiome on plant-derived carbon in 32-year long-term inorganic and organic fertilized soils (2015, methodology): “1 ml quick-seal polyallomer tubes in a VTi 90 vertical rotor (Beckman Coulter, CA, USA), the rhizosphere DNA was fractionated by the cesium chloride (CsCl) equilibrium density gradient centrifugation (Lueders et al., 2004).”
• Bacteria rather than Archaea dominate microbial ammonia oxidation in an agricultural soil. (2009, methodology): “72 g ml-1 subjected to centrifugation at 177 000 g for 44 h at 20°C (Lueders et al., 2004; Freitag et al., 2006).”
• Characterization of methanogenic Archaea and stable isotope fractionation during methane production in the profundal sediment of an oligotrophic lake (Lake Stechlin, Germany) (2007, methodology): “Almost full-length rRNA gene amplicons of Escherichia coli and Methanosarcina barkeri were used as standards for Bacteria and Archaea quantification, respectively (Lueders et al. 2004).”