Flow cytometric determination of lipid content in a marine dinoflagellate, Crypthecodinium cohnii

By A. de la Jara, H. Mendoza, A. Martel, C. Molina, Laurette Nordströn, Vladimir de la Rosa, R. Díaz. Journal of Applied Phycology, 2003

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Isolation of high-lipid content strains of the marine microalga Tetraselmis suecica for biodiesel production by flow cytometry and single-cell sorting (2011, background): “…highly significant correlations between the NR fluorescence and total microalgal lipid content (Chen et al. 2009; Cooksey et al. 1987; de la Jara et al. 2003; Lopes da Silva et al. 2009), thus supporting the view that the dye is a useful proxy for the quantification of lipids in microalgae…”
Improved Nile Red staining of Nannochloropsis sp. (2011, background): “ Cooksey et al. (1987) demonstrated a linear correlation between NR fluorescence and the total lipid content of microalgae, where an increase of lipids in aging algal cells was due primarily to neutral lipids rather than glyco- or phospholipids.”
Estimate by means of flow cytometry of variation in composition of fatty acids from Tetraselmis suecica in response to culture conditions (2010, methodology): “Flow cytometric analysis Cells from different growth conditions were stained with 15 mM NR (Sigma), as described by de la Jara et al. (2003).”
Lipid accumulation and CO2 utilization of Nannochloropsis oculata in response to CO2 aeration. (2009, methodology): “In the method, the microalgal cells were stained with Nile Red (Sigma, St. Louis, MO, USA) followed the protocol reported by de la Jara et al. (2003) .”