Detection of biofilm formation among the clinical isolates of Staphylococci: an evaluation of three different screening methods.

By T. Mathur, Smita Singhal, S. Khan, D. Upadhyay, T. Fatma, A. Rattan. Indian Journal of Medical Microbiology, 2006

741 citations recorded by the source corpus.

PURPOSE The purpose of this study was to evaluate three methods for detection of biofilm formation in staphylococci. METHODS For detection of biofilm formation, 152 clinical isolates of Staphylococcus spp. were screened by tissue culture plate (TCP), Tube method (TM) and Congo red agar (CRA) method. RESULTS Of the 152 Staphylococcus spp. 88(57.8%) displayed a biofilm-positive phenotype under the optimized conditions in the TCP method and strains were further classified as high 22 (14.47 %) and moderate 60 (39.4 %) while in 70 (46.0 %) isolates weak or no biofilm was detected. Though TM correlated well with the TCP test for 18 (11.8 %) strongly biofilm producing strains, weak producers were difficult to discriminate from biofilm negative isolates. Screening on CRA does not correlate well with either of the two methods for detecting biofilm formation in staphylococci. CONCLUSION The TCP method was found to be most sensitive, accurate and reproducible screening method for detection of biofilm formation by staphylococci and has the advantage of being a quantitative model to study the adherence of staphylococci on biomedical devices.

Subfields: Clinical Biochemistry, Infectious Diseases, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e9d0ad65710774644ead7dea095f21c283f7444e

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In Molecular Biology

1. S. Singh
2. I. Cirkovic
3. S. Stepanović

Citations in the corpus

• Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci (2007, methodology): “The duration of incubation is important since the density of biofilm is dependent on incubation time (3, 25, 47).”
• Optimization of microtitre plate assay for the testing of biofilm formation ability in different Salmonella serotypes (2011, background): “In contrast great majority of biofilm studies recommended the incubation for 24 h (Deighton et al., 2001; Moretro et al., 2003; Mathur et al., 2006; Vasudevan et al., 2003; Djordjevic et al., 2002; Stepanovic et al., 2004).”