The Arabidopsis NPR1 protein is a receptor for the plant defense hormone salicylic acid.

By Yue Wu, Di Zhang, Jee Yan Chu, Patrick J Boyle, Yong Wang, I. Brindle, V. De Luca, C. Després. Cell Reports, 2012

769 citations recorded by the source corpus.

Salicylic acid (SA) is an essential hormone in plant immunity, but its receptor has remained elusive for decades. The transcriptional coregulator NPR1 is central to the activation of SA-dependent defense genes, and we previously found that Cys(521) and Cys(529) of Arabidopsis NPR1's transactivation domain are critical for coactivator function. Here, we demonstrate that NPR1 directly binds SA, but not inactive structural analogs, with an affinity similar to that of other hormone-receptor interactions and consistent with in vivo Arabidopsis SA concentrations. Binding of SA occurs through Cys(521/529) via the transition metal copper. Mechanistically, our results suggest that binding of SA causes a conformational change in NPR1 that is accompanied by the release of the C-terminal transactivation domain from the N-terminal autoinhibitory BTB/POZ domain. While NPR1 is already known as a link between the SA signaling molecule and defense-gene activation, we now show that NPR1 is the receptor for SA.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/667322966462d054f607de708bc94f563c903dcd

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Molecular Biology

1. Jian Yao
2. Bethany Huot
3. S. He
4. Beronda L. Montgomery
5. Sergey Shabala
6. Q. Fariduddin
7. M. Janda
8. E. Ruelland

In Plant Science

1. J. Bose
2. Z. Rengel
3. Maheswari Jayakannan
4. Olga Babourina
5. Jian Yao
6. Bethany Huot
7. S. He
8. T. Janda

Citations in the corpus

• Plant hormone-mediated regulation of stress responses (2016, background): “However, recent studies have highlighted that NPR1 is a SA receptor and SA directly regulates the conformation of NPR1 by deoligomerizing NPR1 into a dimer [27].”
• Salicylic acid in plant salinity stress signalling and tolerance (2015, background): “226 Another SA receptor, NPR1 (non-expresser of PR proteins 1) emerged as a master regulatory 227 protein of SA-dependent defence responses by being a transcriptional co-activator of PR-228 gene expression (Vlot et al. 2009; Wu et al. 2012).”
• Growth-defense tradeoffs in plants: a balancing act to optimize fitness. (2014, positive): “After years of searching, two recent studies have proposed NONEXPRESSOR OF PATHOGENESIS RELATED PROTEINS 1 (NPR1) and its paralogs, NPR3 and NPR4, to act as SA receptors (Attaran and He, 2012; Fu et al., 2012; Wu et al., 2012).”
• Multifaceted Role of Salicylic Acid in Combating Cold Stress in Plants: A Review (2020, background): “NPR1 (NONEXPRESSOR OF PATHOGENESISRELATED GENES1) have been shown to play the role of SA receptor (Wu et al. 2012) which interact with the basic leucine zipper transcription factors such as TGACG-BINDING FACTOR5 (TGA5), TGA6 and TGA2 for SA induced PR expression and resistance against pathogens (Zha”
• Engineering plants for heavy metal stress tolerance (2018, positive): “Higher levels of metalloid/ metal toxicity (As, Cd, Mn, Zn and Ni) affect electron passage system on the acceptor side of photosystem II (PS II), thereby inhibiting rate of electron flow between photosystem PS (II) and PS (I) due to toxicity of their ions to membrane lipids (Wu et al. 2012).”
• Magical mystery tour: Salicylic acid signalling (2015, background): “(Wu et al., 2012) The affinity of NPR1 to SA is similar to that of other hormone receptor interactions.”
• Systemic acquired resistance: turning local infection into global defense. (2013, neutral): “(148), or through degradation of a repressor similar to other hormones, such as JA and auxin? Structural studies of NPRs may help us answer these questions.”