Extracellular biosynthesis of silver nanoparticle using Streptomyces sp. 09 PBT 005 and its antibacterial and cytotoxic properties
By P. S. Kumar, Chandrasekar Balachandran, V. Duraipandiyan, D. Ramasamy, S. Ignacimuthu, N. Al-Dhabi. Applied Nanoscience, 2015
89 citations recorded by the source corpus.
The application of microorganisms for the synthesis of nanoparticles as an eco-friendly and promising approach is welcome due to its non-toxicity and simplicity. The aim of this study was to synthesize silver nanoparticle using Streptomyces sp. (09 PBT 005). 09 PBT 005 was isolated from the soil sample of the agriculture field in Vengodu, Thiruvannamalai district, Tamil Nadu, India. 09 PBT 005 was subjected to molecular characterization by 16S rRNA sequence analysis. It was found that 09 PBT 005 belonged to Streptomyces sp. The isolate Streptomyces sp. 09 PBT 005 was inoculated in fermentation medium and incubated at 30 ºC for 12 days in different pH conditions. The 0.02 molar concentration showed good antibacterial activity against Gram-positive and Gram-negative bacteria at pH-7. The synthesis of silver nanoparticles was investigated by UV–Vis spectroscopy, scanning electron microscopy and Fourier Transform Infrared analysis. The synthesized AgNPs sizes were found to be in the dimensions ranging between 198 and 595 nm. The cytotoxicity of the synthesized nanoparticles was studied against A549 adenocarcinoma lung cancer cell line. It showed 83.23 % activity at 100 μl with IC 50 value of 50 μl. This method will be useful in the biosynthesis of nanoparticles.
Subfields: Biomedical Engineering, Materials Chemistry
View Paper: https://www.semanticscholar.org/paper/e9bc97b39fc658d0a3f516fb15161a3e863c9a51
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In Biomedical Engineering
In Materials Chemistry
Citations in the corpus
- Extracellular biosynthesis of silver nanoparticles using Streptomyces griseoplanus SAI-25 and its antifungal activity against Macrophomina phaseolina, the charcoal rot pathogen of sorghum (2018, background): “…from marine sediments of South Korea (Manivasagan et al., 2013), Streptomyces sp. LK3 from marine sediments (Karthik et al., 2014), Streptomyces sp. 09 PBT 005 from sugarcane rhizosphere soil (Saravanakumar et al., 2014) and Streptomyces atrovirens from marine sediments (Subbaiya et al., 2017).”
- A review on biosynthesis of silver nanoparticles and their biocidal properties (2018, background): “In addition, treatment with bacterial Ag NPs has shown the cell viability reduction in a dose-dependent manner in HeLa cervical cancer [278, 279], MDA-MB-231breast cancer [280], A549 adenocarcinoma lung cancer [281] and HEP2 [282] cell lines.”