Genetic transformation of the green alga: Chlamydomonas reinhardtii by Agrobacterium tumefaciens

By Sasidharanpillai Vinod Kumar, R. Misquitta, V. S. Reddy, B. J. Rao, M. Rajam. 2004

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Algal transgenics and biotechnology (2007, background): “2001) and, most surprisingly, in the unicellular green alga Chlamydomonas reinhardtii (Kumar et al. 2004).”
ALGAL TRANSGENICS IN THE GENOMIC ERA 1 (2005, background): “…transformation has been shown to be applicable to a wide range of plant and fungal species (Gelvin 2003) and has now been used to transform Porphyra (Cheney 2001), the giant unicellular alga Acetabularia acetabulum (D. Mandoli personal communication) and C. reinhardtii (Kumar et al. 2004).”
Trends and novel strategies for enhancing lipid accumulation and quality in microalgae (2016, methodology): “widely used technique for plants [108,110] due its natural ability to”
Genetic Engineering of Algae for Enhanced Biofuel Production (2010, methodology): “This problem could be circumvented by the introduction of an AGPase that does not require 3-PGA, such as the Mos(1-198)/SH2 AGPase, which still has activity even without the presence of an activator (14).”
Establishment of Agrobacterium tumefaciens-mediated genetic transformation in Dunaliella bardawil (2011, unknown): “, 2004 ) and Haematococcus pluvialis (Kathiresan et al.”
A green light for engineered algae: redirecting metabolism to fuel a biotechnology revolution. (2008, background): “Through genetic manipulation of the bacterium’s TDNA (transfer DNA), tumor-inducing genes can be replaced with useful gene constructs, which can then be introduced into microalgal cells [45].”