Ribosome mediated specificity in Hox mRNA translation and vertebrate tissue patterning

By N. Kondrashov, A. Pusic, C. Stumpf, Kunihiko Shimizu, A. Hsieh, J. Ishijima, T. Shiroishi, M. Barna. Cell, 2011

594 citations recorded by the source corpus.

Historically, the ribosome has been viewed as a complex ribozyme with constitutive rather than regulatory capacity in mRNA translation. Here we identify mutations of the Ribosomal Protein L38 (Rpl38) gene in mice exhibiting surprising tissue specific patterning defects, including pronounced homeotic transformations of the axial skeleton. In Rpl38 mutant embryos, global protein synthesis is unchanged however the translation of a select subset of Homeobox mRNAs is perturbed. Our data reveal that RPL38 facilitates 80S complex formation on these mRNAs as a regulatory component of the ribosome to confer transcript-specific translational control. We further show that Rpl38 expression is markedly enriched in regions of the embryo where loss-of-function phenotypes occur. Unexpectedly, a ribosomal protein (RP) expression screen reveals dynamic regulation of individual RPs within the vertebrate embryo. Collectively, these findings suggest that RP activity may be highly regulated to impart a new layer of specificity in the control of gene expression and mammalian development.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/e94c05d7763c9fe48388448c2248d855f4b7e895

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In Molecular Biology

Citations in the corpus

Ribosomal proteins: functions beyond the ribosome. (2015, background): “…S27L, S29, L24, L27A, L29, L38, LP1 Miller et al. (2003), Kirn-Safran et al. (2007), Barkic et al. (2009), Oristian et al. (2009), Duan et al. (2011), Kondrashov et al. (2011), Terzian et al. (2011), Taylor et al. (2012), Watkins-Chow et al. (2013), Perucho et al. (2014) and Xiong et al.…”