Rapid screening method for lipid production in alga based on Nile red fluorescence

By Guanhua Huang, Gu Chen, Feng Chen. 2009

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The effects of nutritional restriction on neutral lipid accumulation in Chlamydomonas and Chlorella (2011, unknown): “Nile red signals were captured using an excitation wavelength of 480 nm, and emission was collected between 560 and 600 nm (Huang et al., 2009; Chen et al., 2009).”
Glycerol and neutral lipid production in the oleaginous marine diatom Phaeodactylum tricornutum promoted by overexpression of glycerol-3-phosphate dehydrogenase (2014, methodology): “[29], the mixture was vortexed for 2 min and centrifuged at 8,000 g for 4 min.”
A high throughput Nile red fluorescence method for rapid quantification of intracellular bacterial polyhydroxyalkanoates (2013, methodology): “Nile red fluorescence method has also been applied in isolating strains which are capable of producing neutral lipids, the quantification of hydrophobic proteins and emulsan [6,10-12].”