Statistical analysis of enzyme kinetic data.

By W. Cleland. Methods in Enzymology, 2006

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One hundred years of Michaelis–Menten kinetics☆ (2015, methodology): “It seems quite unlikely that this would have been the case if the original analysis had been made in terms of the hyperbola, as it was not until much later that satisfactory methods for direct fitting of the hyperbola were introduced (Cleland, 1967; Johansen and Lumry, 1961; Wilkinson, 1961).”
An EXCEL template for calculation of enzyme kinetic parameters by non-linear regression (1998, methodology): “The advantages of using non-linear regression instead of linearization of the model have been documented extensively (Suelter, 1985; Tommasini et al., 1985; Henderson, 1992).”
Fitting Straight Lines with Replicated Observations by Linear Regression: The Least Squares Postulates (2004, background): “The same considerations are applied in enzyme kinetics (112), the precision with which the substrate concentrations are known depends on the accuracy of the pipetting in making up the reaction mixtures and, if different dilutions of one stock solution are used and care is taken, the resultant random”