Rapid blue light induction of protein interactions in living cells

By M. Kennedy, R. Hughes, Leslie A. Peteya, J. Schwartz, Michael D. Ehlers, C. Tucker. Nature Methods, 2010

776 citations recorded by the source corpus.

Dimerizers allowing inducible control of protein-protein interactions are powerful tools for manipulating biological processes. Here we describe genetically encoded light-inducible protein-interaction modules based on Arabidopsis thaliana cryptochrome 2 and CIB1 that require no exogenous ligands and dimerize on blue-light exposure with subsecond time resolution and subcellular spatial resolution. We demonstrate the utility of this system by inducing protein translocation, transcription and Cre recombinase–mediated DNA recombination using light.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/e66dc6c679bf8b6d79b7ea47bea23f1eaf7e9456

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In Molecular Biology

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Citations in the corpus

• Optical Control of Mammalian Endogenous Transcription and Epigenetic States (2013, methodology): “The second component includes CRY2’s interacting partner CIB1 6,15 fused to a desired effector domain (CIB1-effector).”