Ploidy level determination within the context of in vitro breeding

By S. Ochatt, E. M. Patat-Ochatt, A. Moessner. Plant Cell Tissue and Organ Culture, 2011

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Induction and identification of tetraploids using in vitro colchicine treatment of Gerbera jamesonii Bolus cv. Sciella (2011, methodology): “The assessment of induced variation was performed by the aid of flow cytometry which proved to be an efficient tool to detect the ploidy level of a plant, as reported (Dickson et al. 1992; Dolezel et al. 2007; Ochatt et al. 2011).”
In vitro induction of polyploidy in Centella asiatica (L.) Urban (2011, methodology): “At the 3rd month of growth following the colchicine treatment, all of the explants treated with colchicine demonstrated lower survival rates than those of the control (Table 1). The ploidy level of the surviving plants was determined by flow cytometry and chromosome count recommended by Ochatt et a”