Adiponectin promotes adipocyte differentiation, insulin sensitivity, and lipid accumulation Published, JLR Papers in Press, April 16, 2005. DOI 10.1194/jlr.M400373-JLR200

By Yu-chang Fu, N. Luo, Richard L. Klein, W. T. Garvey. Journal of Lipid Research, 2005

651 citations recorded by the source corpus.

Adiponectin is secreted from adipocytes, and low circulating levels have been epidemiologically associated with obesity, insulin resistance, type 2 diabetes, and cardiovascular disease. To investigate whether adiponectin could exert autocrine effects in adipocytes, we expressed the adiponectin gene in 3T3-L1 fibroblasts. We observed that 3T3-L1 fibroblasts expressing adiponectin have a fast growth phase and reach confluence more rapidly compared with control cells or LacZ-transduced cells. Furthermore, cells with overexpressed adiponectin were observed to differentiate into adipocytes more rapidly, and during adipogenesis, they exhibited more prolonged and robust gene expression for related transcriptional factors, CCAAT/enhancer binding protein α (C/EBP2), peroxisome proliferator-activated receptor γ (PPARγ), and adipocyte determination and differentiation factor 1/sterol-regulatory element binding protein 1c (ADD1/SREBP1c) and earlier suppression of PPARγ coactivator-1α (PGC-1α). In fully differentiated adipocytes, adiponectin-overexpressing cells accumulated more and larger lipid droplets compared with control cells. Also, adiponectin increased insulin's ability to maximally stimulate glucose uptake by 78% through increased glucose transporter 4 (GLUT4) gene expression and increased GLUT4 recruitment to the plasma membrane. These data suggest a new role for adiponectin as an autocrine factor in adipose tissues: promoting cell proliferation and differentiation from preadipocytes into adipocytes, augmenting programmed gene expression responsible for adipogenesis, and increasing lipid content and insulin responsiveness of the glucose transport system in adipocytes.

Subfields: Epidemiology, Molecular Biology, Physiology

View Paper: https://www.semanticscholar.org/paper/e571453b8f6ed117c545ce94a454f9343ebb3c8d

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Epidemiology

1. P. Scherer
2. Sushil Kumar Jain
3. A. Achari
4. Haiming Cao
5. M. Blüher

In Molecular Biology

1. P. Scherer
2. Sushil Kumar Jain
3. A. Achari

In Physiology

1. J. Rutkowski
2. Sushil Kumar Jain
3. A. Achari
4. P. Scherer
5. Haiming Cao
6. M. Blüher

Citations in the corpus

• Adiponectin, a Therapeutic Target for Obesity, Diabetes, and Endothelial Dysfunction (2017, background): “In adipocytes, C/EBPα, PPARγ, and sterol regulatory element-binding protein (SREBP)-1c are involved in promoting adipogenesis, and increasing lipid content and insulin directed glucose transport [89].”
• Adiponectin, Leptin, and Fatty Acids in the Maintenance of Metabolic Homeostasis Through Adipose Tissue Crosstalk (2016, background): “Given themore recent findings of Soedling and colleagues, it is possible that the effects of leptin on insulin secretion are mediated by leptin signaling in the brain (Fujikawa et al., 2013) (Figure 3).”