Chemical conversion of nitrate and nitrite to nitrous oxide for nitrogen and oxygen isotopic analysis in freshwater and seawater.

By M. McIlvin, M. Altabet. Analytical Chemistry, 2005

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Nitrification and ammonium dynamics in Taihu Lake, China: seasonal competition for ammonium between nitrifiers and cyanobacteria (2017, methodology): “25 mL of 1 : 1 (v : v) 2 M NaN3 : 20 % CH3COOH solution (previously purged with Ar for 30 min), followed by incubation for 1 h at 30 C (McIlvin and Altabet, 2005).”
Nitrification in Marine Systems (2008, positive): “Although they have not yet been widely applied for this purpose, the recently introduced sensitive isotope methods for determination of N content of NO2 and NO3 (McIlvin and Altabet, 2005; Sigman et al., 2001) should make true tracer level incubations possible.”
Depth distribution of ammonia oxidation rates and ammonia‐oxidizer community composition in the Sargasso Sea (2013, methodology): “Analysis of NO{2 —The sodium azide method of McIlvin and Altabet (2005) was used to convert NO{2 to N2O for 15N analysis.”
Revising the nitrogen cycle in the Peruvian oxygen minimum zone (2009, methodology): “In the same 15N incubations, the rates of nitrate reduction and aerobic ammonia oxidation were determined as net 15NO2 production in the 15NO3 and 15NH4 14NO2 incuba- tions, respectively, measured after conversion to N2 (13) or N2O (30).”