The ability of clove oil and MS‐222 to minimize handling stress in rainbow trout (Oncorhynchus mykiss Walbaum)

By G. N. Wagner, T. D. Singer, R. Mckinley. 2003

171 citations recorded by the source corpus.

The effects of 60-mg L-1 clove oil and 60-mg L-1 tricaine methanesulphonate (MS-222) on the blood chemistry of rainbow trout were compared after exposure to handling stress via caudal puncture blood sampling. Fish sampled by caudal puncture and subsequently exposed to anaesthetics showed a typical handling stress response over a 48-h period. There were no significant differences between the responses of fish exposed to equal concentrations of clove oil and MS-222, with the following exceptions: the blood glucose at full anaesthesia, and lactate at full recovery increased significantly in the clove oil-exposed fish. In a subsequent experiment, the stress response observed in fish sampled by caudal puncture and exposed to clove oil and MS-222 was compared with a non-anaesthetized control group. The increases in plasma cortisol levels were significantly lower at recovery in fish treated with either anaesthetic compared with the control fish. Fish exposed to MS-222 had significantly higher cortisol levels at 1 h. These findings show that few differences exist between the anaesthetic effects of clove oil and MS-222 on the physiological response of fish to stress. However, clove oil is more effective at reducing the short-term stress response induced by handling and blood sampling, and is recommended as an alternative fish anaesthetic.

Subfields: Aquatic Science, Ecology, Immunology

View Paper: https://www.semanticscholar.org/paper/e50cb164fc7492f74f961a61619731940aa94e90

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Citations in the corpus

Cortisol and Glucose: Reliable indicators of fish stress? (2009, background): “To this respect, it has been demonstrated that catecholamines itself can increase sugar levels (Wagner et al. 2003).”
Physiological response of wild rainbow trout to angling: Impact of angling duration, fish size, body condition, and temperature (2005, background): “A recent study found that rainbow trout handled and subsequently anesthetized with MS-222 or clove oil experienced a lower lactate response and suppressed cortisol response compared with fish handled without anesthesia (Wagner et al., 2003).”
Behavioral and physiological assessment of low concentrations of clove oil anaesthetic for handling and transporting largemouth bass (Micropterus salmoides) (2004, background): “…disturbances and mitigative effects between clove oil and MS 222 (e.g., Cho and Heath, 2000; Iversen et al., 2003; Pirohen and Schreck, 2003; Wagner et al., 2003), we suggest that clove oil should be an effective alternative transport anaesthetic, especially when combined with some of the…”
A review of tricaine methanesulfonate for anesthesia of fish (2010, background): “It is recommended that stages required for surgery, like Stage 4, should be achieved in less than 3 min (Wedemeyer et al. 1990; Wedemeyer 1997; Mommsen et al. 1999; Wagner et al. 2003).”
Effects of clove oil and MS‐222 on blood hormone profiles in rainbow trout Oncorhynchus mykiss, Walbaum (2004, methodology): “Althoughmany studies have evaluated the relative anaesthetic e¡ects of clove oil and MS-222 on ¢sh, few, to date, have evaluated the associated physiological impacts of these anaesthetics (Prince & Powell 2000;Wagner et al. 2003).”