CRISPR-Cas9 Structures and Mechanisms.

By F. Jiang, Jennifer A. Doudna. Annual Review of Biophysics, 2017

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How researchers see this paper

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The Pangenome: Diversity, Dynamics and Evolution of Genomes (2020, background): “A complex formed by Cas proteins and crRNA leads to the degradation of invading foreign nucleic acid, protecting cells from future invasion (Jiang and Doudna 2017; Adli 2018).”
The Biology of CRISPR-Cas: Backward and Forward. (2018, neutral): “…in inactive and active nu-cleic-acid-bound states, together with numerous biochemical studies, have substantially contributed to our understanding of the interference mechanism in type II systems and are the subject of several in-depth reviews (Jiang and Doudna, 2017; van der Oost et al., 2014).”
CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. (2019, background): “Ablation of catalytic residues in either domain produces a DNA nickase (nCas9), while, importantly, the inactivation of both domains produces a deactivated Cas9 protein (dCas9) that can still target specific genomic loci and serve as a scaffold for recruiting effector proteins (52).”
Molecular targeted therapy: Treating cancer with specificity (2018, background): “This technology allows the elucidation of the gene function involved in disease development and progression, correction of disease-causing mutations, manipulation of oncogenes or tumour suppressor genes, as well as the identification of drug-target and disease-resistance genes (Jiang and Doudna, 201”