Isolation and Characterization of a Bacteriophage Preying an Antifungal Bacterium

By Aryan Rahimi-Midani, Kyoung-Ho Kim, Seon-Woo Lee, S. Jung, T. Choi. Plant Pathology Journal, 2016

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Several Bacillus species were isolated from rice field soils, and 16S rRNA gene sequence analysis showed that Bacillus cereus was the most abundant. A strain named BC1 showed antifungal activity against Rhizoctonia solani. Bacteriophages infecting strain BC1 were isolated from the same soil sample. The isolated phage PK16 had an icosahedral head of 100 ± 5 nm and tail of 200 ± 5 nm, indicating that it belonged to the family Myoviridae. Analysis of the complete linear dsDNA genome revealed a 158,127-bp genome with G + C content of 39.9% comprising 235 open reading frames as well as 19 tRNA genes (including 1 pseudogene). Blastp analysis showed that the proteins encoded by the PK16 genome had the closest hits to proteins of seven different bacteriophages. A neighbor-joining phylogenetic tree based on the major capsid protein showed a robust clustering of phage PK16 with phage JBP901 and BCP8-2 isolated from Korean fermented food.

Subfields: Ecology, Endocrinology, Plant Science

View Paper: https://www.semanticscholar.org/paper/e45c85e361e6baa3f87d026143c20e2a8584b97b

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