A Simple Method for Developing Microsatellite Markers using Amplified Fragments of Inter-simple Sequence Repeat (ISSR)

By C. Lian, Zhihua Zhou, T. Hogetsu. Journal of plant research, 2001

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Methods for Development of Microsatellite Markers: An Overview (2014, unknown): “The ‘dual suppression method’ (Lian et al., 2001; Lian and Hogetsu, 2002) involved cloning and sequencing of ISSR amplicons and initiating a nested PCR (with primers ‘IP1’- designed from the region flanking the microsatellite sequence and ‘IP2’- sequence between IP1 and the microsatellite sequence).”
Non-coding nuclear DNA markers in phylogenetic reconstruction (2009, methodology): “Additionally, inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) were also used for the isolation of microsatellites (Wu et al. 1994, Lian et al. 2001).”
Inter-simple sequence repeat (ISSR) markers: are we doing it right? (2015, background): “…author (W.L. Ng, e-mail: ng.wl85@gmail.com) locus co-dominant ‘sequence characterised amplified region (SCAR)’ markers (e.g. Paran & Michelmore 1993), and ISSR for the development of single-locus co-dominant microsatellite markers (e.g. Fisher et al. 1996; Lian et al. 2001; Adibah et al. 2012).”