An optimized fluorescent probe for visualizing glutamate neurotransmission
By J. Marvin, B. Borghuis, Lin Tian, Joseph Cichon, Mark T. Harnett, Jasper Akerboom, A. Gordus, S. Renninger, Tsai-Wen Chen, Cori Bargmann and 6 more. 2013
942 citations recorded by the source corpus.
We describe an intensity-based glutamate-sensing fluorescent reporter (iGluSnFR) with signal-to-noise ratio and kinetics appropriate for in vivo imaging. We engineered iGluSnFR in vitro to maximize its fluorescence change, and we validated its utility for visualizing glutamate release by neurons and astrocytes in increasingly intact neurological systems. In hippocampal culture, iGluSnFR detected single field stimulus–evoked glutamate release events. In pyramidal neurons in acute brain slices, glutamate uncaging at single spines showed that iGluSnFR responds robustly and specifically to glutamate in situ, and responses correlate with voltage changes. In mouse retina, iGluSnFR-expressing neurons showed intact light-evoked excitatory currents, and the sensor revealed tonic glutamate signaling in response to light stimuli. In worms, glutamate signals preceded and predicted postsynaptic calcium transients. In zebrafish, iGluSnFR revealed spatial organization of direction-selective synaptic activity in the optic tectum. Finally, in mouse forelimb motor cortex, iGluSnFR expression in layer V pyramidal neurons revealed task-dependent single-spine activity during running.
Subfields: Cellular and Molecular Neuroscience, Molecular Biology
How researchers see this paper
The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.
In Cellular and Molecular Neuroscience
- Viviana Gradinaru
- Ed Boyden
- D. Dombeck
- Lin Tian
- Fritjof Helmchen
- Hongkui Zeng
- Matteo Carandini
- Bosiljka Tasic
In Molecular Biology
- Bosiljka Tasic
- Ed Boyden
- Fritjof Helmchen
- Hongkui Zeng
- A. Nagy
- Susan Sunkin
- Matteo Carandini
- L. Looger
Citations in the corpus
- Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors (2018, methodology): “High-quality single fluorescence protein (FP)-based sensors that report calcium or glutamate transients with subsecond temporal resolution have recently been developed and are widely used (18, 19).”
- Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance (2015, positive): “…indicators GCaMP6f and GCaMP6s (Chen et al., 2013), voltage indicator VSFP-Butterfly 1.2 (VSFPB) (Akemann et al., 2012), glutamate sensor iGluSnFR (Marvin et al., 2013), and red-light optogenetic inhibitor Jaws (Chuong et al., 2014), for further direct comparison between the Rosa-CAG and…”
- A gut-brain neural circuit for nutrient sensory transduction (2018, background): “This membrane-bound protein fluoresces green in the presence of glutamate (27).”
- Genetically encoded calcium indicators for multi-color neural activity imaging and combination with optogenetics (2013, methodology): “…proline in the first linker of B-GECO1, GEM-GECO1, and R-GECO1 is consistent with our results for GCaMP (Akerboom et al., 2012a) and bacterial periplasmic binding protein-based sensors for maltose (Marvin et al., 2011), organophosphorous (Alicea et al., 2011), and glutamate (Marvin et al., 2013).”