The role of collagen in bone apatite formation in the presence of hydroxyapatite nucleation inhibitors
By F. Nudelman, Koen Pieterse, Anne George, P. Bomans, Heiner Friedrich, Laura J. Brylka, P. A. Hilbers, G. de With, Nico A.J.M. Sommerdijk. Nature Materials, 2010
1,107 citations recorded by the source corpus.
Bone is a composite material, in which collagen fibrils form a scaffold for a highly organized arrangement of uniaxially oriented apatite crystals1,2. In the periodic 67 nm cross-striated pattern of the collagen fibril3–5, the less dense 40-nm-long gap zone has been implicated as the place where apatite crystals nucleate from an amorphous phase, and subsequently grow6–9. This process is believed to be directed by highly acidic non-collagenous proteins6,7,9–11; however, the role of the collagen matrix12–14 during bone apatite mineralization remains unknown. Here, combining nanometre-scale resolution cryogenic transmission electron microscopy and cryogenic electron tomography15 with molecular modelling, we show that collagen functions in synergy with inhibitors of hydroxyapatite nucleation to actively control mineralization. The positive net charge close to the C-terminal end of the collagen molecules promotes the infiltration of the fibrils with amorphous calcium phosphate (ACP). Furthermore, the clusters of charged amino acids, both in gap and overlap regions, form nucleation sites controlling the conversion of ACP into a parallel array of oriented apatite crystals. We developed a model describing the mechanisms through which the structure, supramolecular assembly and charge distribution of collagen can control mineralization in the presence of inhibitors of hydroxyapatite nucleation.
Subfields: Biomaterials, Biomedical Engineering, Rheumatology
View Paper: https://www.semanticscholar.org/paper/e3f30db324b569432e95b8da5981ebc76b1351e3
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Citations in the corpus
- Demineralization–remineralization dynamics in teeth and bone (2016, neutral): “9 Pathological processes of mineralization result in calcification within blood vessels or in, for example, the kidney or formation of gallstones.”
- Characterization of Bone and Bone-Based Graft Materials Using FTIR Spectroscopy (2012, neutral): “2) (Nudelman et al., 2010).”
- Crystallization by particle attachment in synthetic, biogenic, and geologic environments (2015, background): “In fact, macromolecules, particularly those that are acidic such as polyacrylic acid and aspartic and glutamic acid-rich (poly)peptides and proteins, can dramatically increase induction times (98), stabilize amorphous precursors (97, 99), induce formation of dense liquid phases (21, 100) and modify ”
- Limitations in Bonding to Dentin and Experimental Strategies to Prevent Bond Degradation (2011, methodology): “…al., 2004) was used as a sequestration agent to simulate the aspartic acid and serine-rich mineralization-promoting C-terminal domain (ASARM) of cleaved DMP-1 (Gericke et al., 2010) to stabilize amorphous calcium phosphate into fluidic nanoprecursor particles (Gower, 2008; Nudelman et al., 2010).”