Kinetic characterization of purified laccase produced from Trametes versicolor IBL-04 in solid state bio-processing of corncobs
By M. Asgher, Hafiz M. N. Iqbal, M. Asad. BioResources, 2012
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A locally isolated white rot fungal strain Trametes versicolor IBL-04 produced high laccase activities in solid state bio-processing of corn cobs. Addition of glucose and yeast extract (C: N ratio; 25:1) enhanced laccase synthesis. Addition of Tween-80 and CuSO4 enhanced laccase production to 1012 U/mL under optimized process conditions. Laccase was further purified to 2.89-fold (specific activity of 840 U/mg) by ammonium sulfate fractional precipitation, dialysis, and Sephadex G-100 gel filtration chromatography. The purified laccase had a relative molecular mass of 63 kDa as detected by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE). Best enzyme activity was at pH 5 and 40oC. Using 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) as substrate, the enzyme showed maximum activity (Vmax) of 780 U/mL with a corresponding Michaelis constant (Km) value of 73µM. Among the different activators/inhibitors, Cu2+, Mn2+, and Fe2+ stimulated laccase activity, whereas EDTA and cystein inhibited the enzyme. The higher Vmax and lower Km for T. versicolor IBL-04laccase as compared to most of the reported laccases suggests its potential for industrial applications.
Subfields: Biotechnology, Health, Toxicology and Mutagenesis, Plant Science
View Paper: https://www.semanticscholar.org/paper/9306cfd4642ff8a5a665374c8d68b3ac8a15c577
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Citations in the corpus
- Advances in the Valorization of Lignocellulosic Materials by Biotechnology: An Overview (2013, background): “…microorganisms, mainly white rot fungi (Baldrian and Gabriel, 2003; Ander and Eriksson 2006; Asgher et al. 2008; Sánchez, 2009; Alvira et al. 2010; Asgher et al. 2012a,b,c; Asgher and Iqbal 2013; Iqbal and Asgher 2013), have been isolated and identified in recent years, and this list still…”
- Advances in lignocellulosic biotechnology: A brief review on lignocellulosic biomass and cellulases (2014, background): “Many other industrially important enzymes produced by other fungi such as Trametes versicolor, Trichoderma, Aspergillus and Rhizopus species have also been extensively studied by several researchers [24-29].”