Isolation and characterization of Pseudomonas resistant to heavy metals contaminants

By H. Hussein, H. Moawad, S. Farag. Arab journal of biotechnology, 2004

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In this work, chromium, copper, nickel, cadmium were selected as a model for metal contamination. This selection is based on the fact that these metals are discharged in many of the industries such as electroplating, detergents, oil refining and others. The isolation of bacteria resistant to different metal ions was done by using mixed industrial and domestic wastewater from the western station of sewage treatment plant in Alexandria. Iron limiting Casamino acid media is used in this study, since it can induce the production of fluorescent siderofores of the Pseudomonas species. Eighteen colonies were selected and purified as single colonies. The preliminary observation and the biochemical identification of these isolates indicated that the selected isolates are belonging to Pseudomonas species. Screening of the bacterial isolates for metal resistance against Cr(VI), Cu(II), Cd(II) and Ni(II) was done by the use of MIC and MTC (Maximum tolerable concentrations). Different metal concentrations were used throughout the screening to select bacterial isolates capable to grow and resist the metal toxicity. The optimum pH of metal precipitation was around 6. Whereas the optimum growth pH for Cr and Ni resistant strains was 5.5, while was 6 for Cu and Cd resistant strains.

Subfields: Environmental Engineering, Health, Toxicology and Mutagenesis, Water Science and Technology

View Paper: https://www.semanticscholar.org/paper/e29aecae99ad2648acbfdc599c4dad83c2eec478

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In Environmental Engineering

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In Water Science and Technology

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