AXOR12, a Novel Human G Protein-coupled Receptor, Activated by the Peptide KiSS-1*
By A. Muir, L. Chamberlain, N. Elshourbagy, D. Michalovich, Darren Moore, A. Calamari, P. Szekeres, H. Sarau, J. Chambers, P. Murdock and 12 more. Journal of Biological Chemistry, 2001
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A novel human G protein-coupled receptor named AXOR12, exhibiting 81% homology to the rat orphan receptor GPR54, was cloned from a human brain cDNA library. Heterologous expression of AXOR12 in mammalian cells permitted the identification of three surrogate agonist peptides, all with a common C-terminal amidated motif. High potency agonism, indicative of a cognate ligand, was evident from peptides derived from the gene KiSS-1, the expression of which prevents metastasis in melanoma cells. Quantitative reverse transcriptase-polymerase chain reaction was used to study the expression of AXOR12 and KiSS-1 in a variety of tissues. The highest levels of expression of AXOR12 mRNA were observed in brain, pituitary gland, and placenta. The highest levels ofKiSS-1 gene expression were observed in placenta and brain. A polyclonal antibody raised to the C terminus of AXOR12 was generated and used to show localization of the receptor to neurons in the cerebellum, cerebral cortex, and brainstem. The biological significance of these expression patterns and the nature of the putative cognate ligand for AXOR12 are discussed.
Subfields: Cellular and Molecular Neuroscience, Molecular Biology, Reproductive Medicine
View Paper: https://www.semanticscholar.org/paper/e16722ca897920132430515411ea975874522fb1
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In Reproductive Medicine
Citations in the corpus
- Kisspeptins and reproduction: physiological roles and regulatory mechanisms. (2012, background): “Although the mapping of the signaling pathways employed by GPR54 was initiated already in 2001 by the use of different heterologous cell systems (228, 292, 318), this aspect has received far less attention than the characterization of other facets of kisspeptin physiology and remains to date partial”
- Hypothalamic–pituitary–adrenal and hypothalamic–pituitary–gonadal axes: sex differences in regulation of stress responsivity (2017, background): “, 2006) and the kisspeptin receptor GPR54 is expressed in GnRH neurons (Clements et al., 2001; Kotani et al., 2001; Muir et al., 2001; Ohtaki et al., 2001).”