The effect of dilution and the use of a post-extraction nucleic acid purification column on the accuracy, precision, and inhibition of environmental DNA samples

By A. McKee, Stephen F. Spear, Todd W. Pierson. 2015

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Environmental DNA - An emerging tool in conservation for monitoring past and present biodiversity (2015, background): “River Crustacean, mussel Switzerland Present Pilliod et al. (2014) Factors influencing detection of eDNA from a streamdwelling amphibian Streams Amphibian (idaho giant salamander) Idaho, USA Present Tréguier et al. (2014) Environmental DNA surveillance for invertebrate species: advantages and techni”
Evaluation of Filtration and DNA Extraction Methods for Environmental DNA Biodiversity Assessments across Multiple Trophic Levels (2017, methodology): “To account for the presence of inhibitory factors in seawater, DNA extracts were subsequently purified using One-step PCR inhibitor removal columns (Zymo Research; McKee et al., 2015).”
Towards the Optimization of eDNA/eRNA Sampling Technologies for Marine Biosecurity Surveillance (2021, background): “, PowerWater or other inhibitor removal kits, [150,155]), or the use of downstream polymerase enzymes that are resistant to inhibition.”