Pressure and cold shock induction of meiotic gynogenesis and triploidy in the European sea bass, Dicentrarchus labrax L.: relative efficiency of methods and parental variability

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Welfare Considerations of Triploid Fish (2012, background): “This is particularly important from an animal welfare perspective, as unacceptably high mortality and deformity rates are caused by inefficient triploid induction procedures (e.g., Peruzzi and Chatain, 2000).”
Cold shock and fish (2008, background): “Peruzzi & Chatain (2000) induced triploidy in the European sea bass Dicentrarchus labrax L. by altering the timing, intensity and duration of cold temperature applications.”
Sex Control in Fish: Approaches, Challenges and Opportunities for Aquaculture (2015, unknown): “Thus protocols need to be individually optimized and precise in their application, as variation often leads to decreased percentages of triploidy induction success [120,121].”